How do you test for indican?
Urine sample must be the first morning urine sample. It is important to use the first morning urine, as indican levels can reduce throughout the day and you want to assess urinary indican levels when they are at their highest.
What color indicates a high positive indican test?
6. Compare the color in the bottom chloroform layer with the Indican Color Chart below. Record results and dispose the materials properly. Clear or Urine color 0: Normal Light Blue 1: Low Positive Blue 2: Medium Positive Violet 3: High Positive Jet Black 4: Very High Positive.
What is indican random urine?
INDICAN RANDOM URINE (National) Test Overview : Used as an aid in the diagnosis of small bowel (intestine) malabsorptive disorders associated with certain bacterial infestation.
What is the other name of indican?
Alternative name: potassium indoxylsulphate; chemical formula C₈H₆NOSO₂OH. ‘We have studied the effect of indican on six commercial procedures for the measurement of total bilirubin in serum. ‘
How is indican detected in urine?
The urinary Indican test is an accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. Note: For research use only. Not intended for diagnostic use. (The Indican Reagent is corrosive.
How is indican formed?
Indican is produced from tryptophan in a manner reminiscent of the production of p-cresol sulfate from tyrosine and phenylalanine. Gut bacteria convert tryptophan to indole, which is then oxidized to indoxyl and conjugated with sulfate in the liver.
What is indican blood test?
The urinary indican test (or Obermeyer test) is an efficient aid used by naturopathic doctors in the screening for bacterial links to digestive issues. In humans after food has been processed into chyme in the stomach, it enters the small intestine.
How is indican made?
Indican is an indole produced when bacteria in the intestine act on the amino acid, tryptophan. Most indoles are excreted in the feces. The remainder is absorbed, metabolized by the liver, and excreted as indicanin the urine.
What is the significance of indican?
Indican is a tryptophan metabolite that is excreted mostly in the feces but also in small amounts in the urine as a result of absorption and detoxification of indole produced by bacterial action on tryptophan in the intestines. The presence of indican in the urine indicates amino acid malabsorption.
Is indole acidic or basic?
Like pyrrole, indole is a weak base and also a weak acid. It is polymerized by strong acids and reacts with potassium hydroxide and Grignard reagents.
What conditions may lead to increased concentration of indican in the urine?
Excessive Indican levels are caused by poor digestion, constipation, exposure to excessive chemicals in the water, food, air, or drugs, or yeast/candida, parasites, or abnormal flora in the gut.